Review



polyclonal anti sirt1 antibody upstate  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology polyclonal anti sirt1 antibody upstate
    FIG. 2. Perturbation of <t>SIRT1</t> activity affects secretion of HMW forms of adiponectin. 3T3-L1 adipocytes (4 days) were exposed to either resveratrol (50 M) or nicotinamide (5 mM) in standard DMEM containing 10% FBS for 2 days. The cells were then cultured in fresh DMEM overnight, at which time the medium and total cell layer were harvested for Western blot analysis of intra- and extracellular proteins on reducing (A) and nonreducing (B) SDS-PAGE as outlined in Materials and Methods.
    Polyclonal Anti Sirt1 Antibody Upstate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+anti+sirt1+antibody+upstate/SIRT1+Antibody/10__1128_slash_mcb__02279___06-92-17-24
    Average 96 stars, based on 1770 article reviews
    polyclonal anti sirt1 antibody upstate - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα"

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα

    Journal: Molecular and Cellular Biology

    doi: 10.1128/mcb.02279-06

    FIG. 2. Perturbation of SIRT1 activity affects secretion of HMW forms of adiponectin. 3T3-L1 adipocytes (4 days) were exposed to either resveratrol (50 M) or nicotinamide (5 mM) in standard DMEM containing 10% FBS for 2 days. The cells were then cultured in fresh DMEM overnight, at which time the medium and total cell layer were harvested for Western blot analysis of intra- and extracellular proteins on reducing (A) and nonreducing (B) SDS-PAGE as outlined in Materials and Methods.
    Figure Legend Snippet: FIG. 2. Perturbation of SIRT1 activity affects secretion of HMW forms of adiponectin. 3T3-L1 adipocytes (4 days) were exposed to either resveratrol (50 M) or nicotinamide (5 mM) in standard DMEM containing 10% FBS for 2 days. The cells were then cultured in fresh DMEM overnight, at which time the medium and total cell layer were harvested for Western blot analysis of intra- and extracellular proteins on reducing (A) and nonreducing (B) SDS-PAGE as outlined in Materials and Methods.

    Techniques Used: Activity Assay, Cell Culture, Western Blot, SDS Page

    FIG. 4. Inhibition of SIRT1 expression enhances secretion of HMW adiponectin. 3T3-L1 preadipocytes expressing a control vector or SIRT1 siRNA were differentiated for the indicated number of days, and medium (extracellular) as well as the total cell layer (intracellular) was harvested for Western blot analysis of proteins on reducing (A) or nonreducing (B) SDS-PAGE employing antibodies to the following proteins: SIRT1, PPAR, C/EBP, adiponectin, adipsin, and aP2/FABP4.
    Figure Legend Snippet: FIG. 4. Inhibition of SIRT1 expression enhances secretion of HMW adiponectin. 3T3-L1 preadipocytes expressing a control vector or SIRT1 siRNA were differentiated for the indicated number of days, and medium (extracellular) as well as the total cell layer (intracellular) was harvested for Western blot analysis of proteins on reducing (A) or nonreducing (B) SDS-PAGE employing antibodies to the following proteins: SIRT1, PPAR, C/EBP, adiponectin, adipsin, and aP2/FABP4.

    Techniques Used: Inhibition, Expressing, Control, Plasmid Preparation, Western Blot, SDS Page

    FIG. 6. SIRT1 regulates expression of the ER oxidoreductase Ero1-L. (A) 3T3-L1 preadipocytes were differentiated for the indicated days, and total cell RNA was extracted for RT-PCR analysis of mRNAs corresponding to PPAR, C/EBP, adiponectin, Ero1-L, FABP4/aP2, and GAPDH. (B and C) 3T3-L1 preadipocytes expressing a control vector (-) or a SIRT1 siRNA () were differentiated for 8 days for RT-PCR analysis of mRNAs (B) or for the indicated days for Western blot analysis of intracellular proteins (C). (D) Control and SIRT1 siRNA-expressing 3T3-L1 preadipocytes were differentiated in the absence (Con) or presence of either a PPAR antagonist (Antag; 10 M T0070907) or PPAR agonist (Trog; 5 M troglitazone), and the medium (extracellular) and total cell layer (intracellular) were harvested for Western blot analysis of the indicated proteins.
    Figure Legend Snippet: FIG. 6. SIRT1 regulates expression of the ER oxidoreductase Ero1-L. (A) 3T3-L1 preadipocytes were differentiated for the indicated days, and total cell RNA was extracted for RT-PCR analysis of mRNAs corresponding to PPAR, C/EBP, adiponectin, Ero1-L, FABP4/aP2, and GAPDH. (B and C) 3T3-L1 preadipocytes expressing a control vector (-) or a SIRT1 siRNA () were differentiated for 8 days for RT-PCR analysis of mRNAs (B) or for the indicated days for Western blot analysis of intracellular proteins (C). (D) Control and SIRT1 siRNA-expressing 3T3-L1 preadipocytes were differentiated in the absence (Con) or presence of either a PPAR antagonist (Antag; 10 M T0070907) or PPAR agonist (Trog; 5 M troglitazone), and the medium (extracellular) and total cell layer (intracellular) were harvested for Western blot analysis of the indicated proteins.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Western Blot

    Related Articles

    Activity Assay:

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-L?
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPARγ and anti-C/EBPα (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators ( 37 ).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (..

    Cell Culture:

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-L?
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPARγ and anti-C/EBPα (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators ( 37 ).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (..

    Western Blot:

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-L?
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPARγ and anti-C/EBPα (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators ( 37 ).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (..

    SDS Page:

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-L?
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPARγ and anti-C/EBPα (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators ( 37 ).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (..

    Inhibition:

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-L?
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPARγ and anti-C/EBPα (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators ( 37 ).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (..

    Expressing:

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-L?
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPARγ and anti-C/EBPα (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators ( 37 ).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (..

    Control:

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-L?
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPARγ and anti-C/EBPα (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators ( 37 ).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (..

    Plasmid Preparation:

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-L?
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPARγ and anti-C/EBPα (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators ( 37 ).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).Isolation and Western blot analysis of total cell proteins was performed as outlined previously (21).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (37).

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-L?
    Article Snippet: Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).Isolation and Western blot analysis of total cell proteins was performed as outlined previously ( 21 ).. The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPARγ and anti-C/EBPα (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).. For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators ( 37 ).For analysis of secreted proteins, aliquots of the culture medium were centrifuged at low speed to remove debris and then subjected to standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as above or nonreducing SDS-PAGE as outlined by Kadowaki and collaborators (..



    Similar Products

    polyclonal anti sirt1 antibody upstate  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology polyclonal anti sirt1 antibody upstate
    FIG. 2. Perturbation of <t>SIRT1</t> activity affects secretion of HMW forms of adiponectin. 3T3-L1 adipocytes (4 days) were exposed to either resveratrol (50 M) or nicotinamide (5 mM) in standard DMEM containing 10% FBS for 2 days. The cells were then cultured in fresh DMEM overnight, at which time the medium and total cell layer were harvested for Western blot analysis of intra- and extracellular proteins on reducing (A) and nonreducing (B) SDS-PAGE as outlined in Materials and Methods.
    Polyclonal Anti Sirt1 Antibody Upstate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+anti+sirt1+antibody+upstate/SIRT1+Antibody/10__1128_slash_mcb__02279___06-92-17-24
    Average 96 stars, based on 1 article reviews
    polyclonal anti sirt1 antibody upstate - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    FIG. 2. Perturbation of SIRT1 activity affects secretion of HMW forms of adiponectin. 3T3-L1 adipocytes (4 days) were exposed to either resveratrol (50 M) or nicotinamide (5 mM) in standard DMEM containing 10% FBS for 2 days. The cells were then cultured in fresh DMEM overnight, at which time the medium and total cell layer were harvested for Western blot analysis of intra- and extracellular proteins on reducing (A) and nonreducing (B) SDS-PAGE as outlined in Materials and Methods.

    Journal: Molecular and Cellular Biology

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα

    doi: 10.1128/mcb.02279-06

    Figure Lengend Snippet: FIG. 2. Perturbation of SIRT1 activity affects secretion of HMW forms of adiponectin. 3T3-L1 adipocytes (4 days) were exposed to either resveratrol (50 M) or nicotinamide (5 mM) in standard DMEM containing 10% FBS for 2 days. The cells were then cultured in fresh DMEM overnight, at which time the medium and total cell layer were harvested for Western blot analysis of intra- and extracellular proteins on reducing (A) and nonreducing (B) SDS-PAGE as outlined in Materials and Methods.

    Article Snippet: The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).

    Techniques: Activity Assay, Cell Culture, Western Blot, SDS Page

    FIG. 4. Inhibition of SIRT1 expression enhances secretion of HMW adiponectin. 3T3-L1 preadipocytes expressing a control vector or SIRT1 siRNA were differentiated for the indicated number of days, and medium (extracellular) as well as the total cell layer (intracellular) was harvested for Western blot analysis of proteins on reducing (A) or nonreducing (B) SDS-PAGE employing antibodies to the following proteins: SIRT1, PPAR, C/EBP, adiponectin, adipsin, and aP2/FABP4.

    Journal: Molecular and Cellular Biology

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα

    doi: 10.1128/mcb.02279-06

    Figure Lengend Snippet: FIG. 4. Inhibition of SIRT1 expression enhances secretion of HMW adiponectin. 3T3-L1 preadipocytes expressing a control vector or SIRT1 siRNA were differentiated for the indicated number of days, and medium (extracellular) as well as the total cell layer (intracellular) was harvested for Western blot analysis of proteins on reducing (A) or nonreducing (B) SDS-PAGE employing antibodies to the following proteins: SIRT1, PPAR, C/EBP, adiponectin, adipsin, and aP2/FABP4.

    Article Snippet: The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).

    Techniques: Inhibition, Expressing, Control, Plasmid Preparation, Western Blot, SDS Page

    FIG. 6. SIRT1 regulates expression of the ER oxidoreductase Ero1-L. (A) 3T3-L1 preadipocytes were differentiated for the indicated days, and total cell RNA was extracted for RT-PCR analysis of mRNAs corresponding to PPAR, C/EBP, adiponectin, Ero1-L, FABP4/aP2, and GAPDH. (B and C) 3T3-L1 preadipocytes expressing a control vector (-) or a SIRT1 siRNA () were differentiated for 8 days for RT-PCR analysis of mRNAs (B) or for the indicated days for Western blot analysis of intracellular proteins (C). (D) Control and SIRT1 siRNA-expressing 3T3-L1 preadipocytes were differentiated in the absence (Con) or presence of either a PPAR antagonist (Antag; 10 M T0070907) or PPAR agonist (Trog; 5 M troglitazone), and the medium (extracellular) and total cell layer (intracellular) were harvested for Western blot analysis of the indicated proteins.

    Journal: Molecular and Cellular Biology

    Article Title: Adiponectin Secretion Is Regulated by SIRT1 and the Endoplasmic Reticulum Oxidoreductase Ero1-Lα

    doi: 10.1128/mcb.02279-06

    Figure Lengend Snippet: FIG. 6. SIRT1 regulates expression of the ER oxidoreductase Ero1-L. (A) 3T3-L1 preadipocytes were differentiated for the indicated days, and total cell RNA was extracted for RT-PCR analysis of mRNAs corresponding to PPAR, C/EBP, adiponectin, Ero1-L, FABP4/aP2, and GAPDH. (B and C) 3T3-L1 preadipocytes expressing a control vector (-) or a SIRT1 siRNA () were differentiated for 8 days for RT-PCR analysis of mRNAs (B) or for the indicated days for Western blot analysis of intracellular proteins (C). (D) Control and SIRT1 siRNA-expressing 3T3-L1 preadipocytes were differentiated in the absence (Con) or presence of either a PPAR antagonist (Antag; 10 M T0070907) or PPAR agonist (Trog; 5 M troglitazone), and the medium (extracellular) and total cell layer (intracellular) were harvested for Western blot analysis of the indicated proteins.

    Article Snippet: The antibodies employed in the analysis were as follows: mouse polyclonal antiadiponectin antibody (Affinity BioReagents, Golden, CO), polyclonal anti-SIRT1 antibody (Upstate), anti-PPAR and anti-C/EBP (Santa Cruz Biotechnology), anti-Ero1 polyclonal antibody (Abnova Co., Taiwan, China), and polyclonal anti-aP2 serum (David Benlohr, University of Minnesota).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Western Blot